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Image Search Results
Journal: Nature
Article Title: A satellite repeat-derived piRNA controls embryonic development of Aedes
doi: 10.1038/s41586-020-2159-2
Figure Lengend Snippet: (a,d,e) Northern blot of tapiR1 and 2 in different tissues of adult mosquitoes (a), upon dsRNA-mediated knockdown of individual PIWI genes (d), and upon knockdown of miRNA and siRNA pathway genes (e), or a control dsRNA treatment (dsFLuc and dsRLuc) in Aag2 cells. U6 snRNA or ethidium bromide-stained rRNA serves as loading controls. (b) Western blot analysis of the indicated PIWI proteins before (input) and after immunoprecipitation (IP) used for the small RNA northern blot of panel c. An IP with empty beads serves as negative control. Tubulin was used to control for non-specific binding. (c) Immunoprecipitation of the indicated PIWI proteins from Aag2 cells followed by northern blot analyses for tapiR1 and 2.
Article Snippet: Membranes were blocked for 1 h with 5% milk in PBS-T (137 mM NaCl, 12 mM phosphate, 2.7 mM KCl, pH 7.4, 0.1 % (v/v) Tween-20) and incubated with PIWI (dilution 1:1000) and
Techniques: Northern Blot, Knockdown, Control, Staining, Western Blot, Immunoprecipitation, Negative Control, Binding Assay